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PCR Enzyme


Zamper X-Taq DNA Polymerase, Hot-start Version

It is effective for amplifying relatively long PCR products as well as the size of PCR products that can be amplified in standard PCR. It has 3’-5’ exonuclease activity and is characterized by inhibiting the formation of non-specific products and primer-dimer formation. In addition, gene amplification is possible up to 15 kb in size.

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  • I Features

    • Proof-reading Function
    • Increased fidelity through 3’-5’ exonuclease activity
    • Long-range PCR
    • Effective for amplifying long PCR product
    • Specificity
    • High amplification efficiency and high sensitivity

  • I Specification

    5’→3’ Exonuclease activity Yes Amplicon size Human gDNA ≤ 10 kb
    Lambda DNA ≤ 15 kb
    3’→5’ Exonuclease activity Yes 3’ A or Blunt ends 3’ A
    Hot-start activity Yes

  • I Applications

    Standard PCR, Long-range PCR, RT-PCR, Primer Extension

  • I Experimental Data

     

     

     

  • I Types of Product

    Types Contents Storage temperature
    Standard · X-Taq HS DNA Polymerase (2.5 U/㎕)
    · 10X X-Taq HS Reaction Buffer (with 20 mM MgCl2)
    · 10 mM dNTP Mixture
    -20 ℃
    Premix Tube type · 2X X-Taq HS DNA Polymerase Premix
    (containing dNTPs, MgCl2 and Stabilizers)
    8-strip type

  • I Ordering Information

    Types Cat. No. Item Size
    Standard Z23025 Zamper X- Taq DNA Polymerase,
    Hot-start Version
    250 U (200 rxns)
    Z23051 500 U (400 rxns)
    Z23052 1,000 U (800 rxns)
    Z23055 2,500 U (2000 rxns)
    Premix Tube type Z23002 Zamper X- Taq DNA Polymerase Premix,
    HS Ver., 20 ㎕ Reaction
    1 ㎖×2 ea (200 rxns)
    Z23005 1 ㎖×5 ea (500 rxns)
    8-strip type Z23201 96 tubes
    Z23205 480 tubes

    · Standard reaction type : Total reaction volume of 50 ㎕
    · Premix reaction type : Total reaction volume of 20 ㎕