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PCR Enzyme


Zamper Hot-start Taq DNA Polymerase

By coupling with Anti-Taq Monoclonal Antibody, it is a Taq DNA polymerase that is activated by high-temperature treatment, so the generation of non-specific products can be minimized. Genes can be effectively amplified when the amount of template DNA is small or the template DNA has a complex structure. Because of its high amplification specificity for individual genes, it can be used for multiplex experiments and molecular diagnostic tests that simultaneously amplify multiple genes. Therefore, gene amplification results can be confirmed quickly.

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  • I Features

    • Thermal Stable & Fast
    • Fast and accurate PCR results can be confirmed due to high processivity
    • Specificity
    • Minimization of non-specific amplification
    • Sensitivity
    • In the case of human genomic DNA, gene amplification is possible from 16 pg of template DNA

  • I Specification

    5’→3’ Exonuclease activity Yes Amplicon size ≤ 6 kb
    3’→5’ Exonuclease activity No 3’ A or Blunt ends 3’ A
    Hot-start activity Yes

  • I Applications

    Standard PCR, Colony PCR, Fast PCR, T/A Cloning, Multiplex PCR

  • I Experimental Data

     

     

     

  • I Types of Product

    Types Contents Storage temperature
    Standard · Hot-start Taq DNA Polymerase (5 U/㎕)
    · 10X Hot-start Taq Reaction Buffer (with 25 mM MgCl2)
    · 10 mM dNTP Mixture     		-20 ℃
    Premix Tube type 2X Hot-start Taq DNA Polymerase Premix
    (containing dNTPs, MgCl2, Stabilizers and Loading Dye)
    8-strip type

  • I Ordering Information

    Types Cat. No. Item Size
    Standard Z21051 Zamper Hot-start Taq DNA Polymerase 500 U (400 rxns)
    Z21052 1,000 U (800 rxns)
    Z21055 2,500 U (2,000 rxns)
    Premix Tube type Z21002 Zamper Hot-start Taq DNA Polymerase Premix 1 ㎖×2 ea (200 rxns)
    Z21005 1 ㎖×5 ea (500 rxns)
    Z52001 Zamper Multiplex PCR Premix 1 ㎖×1 ea (100 rxns)
    Z52003 1 ㎖×3 ea (300 rxns)
    Z52005 1 ㎖×5 ea (500 rxns)
    8-strip type Z21201 Zamper Hot-start Taq DNA Polymerase Premix, 20 ㎕ Reaction 96 tubes
    Z21205 480 tubes
    Z52096 Zamper Multiplex PCR Premix, 20 ㎕ Reaction 96 tubes
    Z52480 480 tubes

    · Standard reaction type : Total reaction volume of 50 ㎕
    · Premix reaction type : Total reaction volume of 20 ㎕
    ※ Contact us for dye-free premix products

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