PRODUCTS
cDNA Synthesis Kit
Zamper RT Premix
Zamper Reverse Transcriptase, a key component of this product, is a recombinant M-MLV Reverse Transcriptase, and it is possible to synthesize cDNA stably in a wide temperature range (45~60℃). Therefore, it can be used efficiently for various experiments. Products for synthesizing cDNA from RNA are available in two types. Zamper cDNA Synthesis Kit consists of individual tubes with the components necessary for cDNA synthesis, so researchers can directly mix and use. In addition, Zamper RT Premix is provided in the form of 5X Premix to use conveniently. These products can synthesize cDNA using RNA equivalent to several pg to several μg, and can produce cDNA of up to 10 kb.
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I Features
- Dynamic range
- RNA can be used in a wide range of concentrations
- Productivity
- Synthesis of cDNA up to 10 kb in length
- Flexible reaction temperature
- Wide range of reverse transcription temperatures
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I Specification
Source M-MLV (Recombinant) cDNA length ≤ 10 kb Reaction temperature 45~60 °C Applicable Primers Random Hexamer
Oligo d(T) Primer
Specific Primer -
I Applications
First-strand cDNA Synthesis, Two-step RT-PCR, Two-step RT-qPCR, Primer Extension Assay
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I Experimental Data
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I Types of Product
Types Contents Storage temperature Kit · Oligo d(T)20, · Random Hexamer, · dNTP Mixture,
· DEPC Water, · 5X RT Reaction Buffer, · 20X Enhancer,
· RNase Inhibitor (40 U/μl), · M-MLV Reverse Transcriptase-20 ℃ Premix Tube type · 5X RT Premix
(containing M-MLV Reverse Transcriptase, RT Reaction Buffer,
dNTP Mixture, RNase Inhibitor, Oligo d(T)20, Random Hexamer,
and Stabilizers)
· DEPC Water -
I Ordering Information
Types Cat. No. Item Size Kit Z73050 Zamper cDNA Synthesis Kit, 20 ㎕ Reaction 50 rxns Z73100 100 rxns Z73200 200 rxns Premix Tube type Z73101 Zamper 5X RT Premix 0.4 ml×1 ea (100 rxns) Z73201 0.4 ml×2 ea (200 rxns) Z73501 1 ml×2 ea (500 rxns) · Standard and Premix reaction types : Total reaction volume of 20 ㎕