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One-step qRT-PCR Master Mix


Zamper One-step qRT-PCR Master Mix with SFCgreen®Ⅰ

Zamper One-step qRT-PCR Master Mix with SFCgreen®Ⅰ product is supplied in the form of a master mix that can perform reverse transcription reaction to synthesize cDNA from template RNA and real-time PCR reaction in one tube. Intercalating dye (SFCgreen®Ⅰ) is included in master mix and can be used with Specific primer set. This product contains Zamper Reverse Transcriptase and Zamper Hot-start Taq DNA Polymerase, which are essential for one-step qRT-PCR experiments. This product composed of optimal composition can be applied to various experiments using RNA because it enables fast and stable synthesis of cDNA. Low-copy genes of 50 copies or less can also be detected. Depending on the purpose of the experiment, you can use master mix products applicable to the fast qPCR protocol, so you can quickly check the experiment results. In addition, Dx product lines capable of genetic quantitative analysis from trace amounts of RNA and viral RNA are also provided.

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  • I Features

    • Sensitivity
    • Low-copy gene detection of 10 to 50 copies
    • Convenience
    • As it is provided in the form of master mix, One-step qRT-PCR reaction can be easily performed by adding template RNA and specific primer set

  • I Applications

    Gene Expression Analysis, Fast One-step qRT-PCR

  • I Experimental Data

     

     

     

  • I Types of Product

    Types Contents Storage temperature
    Premix Tube type 2X One-step qRT-PCR Master Mix
    (including SFCgreen®Ⅰ in the mixture)
    -20 ℃

  • I Ordering Information

    Types Cat. No. Item Size
    Premix Tube type Z74010 Zamper 2X One-step qRT-PCR Master Mix
    with SFCgreen®Ⅰ
    1 ㎖×1 ea (100 rxns)
    Z74050 1 ㎖×5 ea (500 rxns)
    Z74310 Zamper 2X Fast One-step qRT-PCR Master Mix
    with SFCgreen®Ⅰ
    1 ㎖×1 ea (100 rxns)
    Z74350 1 ㎖×5 ea (500 rxns)
    Z81010 Zamper DxQ 2X One-step qRT-PCR Master Mix
    with SFCgreen®Ⅰ
    1 ㎖×1 ea (100 rxns)
    Z81050 1 ㎖×5 ea (500 rxns)
    Z82100 Zamper DxL 2X One-step qRT-PCR Master Mix
    with SFCgreen®Ⅰ
    1 ㎖×1 ea (100 rxns)
    Z82500 1 ㎖×5 ea (500 rxns)

    · Total reaction volume is 20 ㎕
    ※ For 4X Master Mix products and products containing UDG, please inquire separately